A RECEPTOR-BINDING SITE AS REVEALED BY THE CRYSTAL STRUCTURE OF CfaE, THE CFA/I FIMBRIAL ADHESIN OF ENTEROTOXIGENIC Escherichia coli
نویسندگان
چکیده
Address correspondence to: Di Xia, Laboratory of Cell Biology, National Cancer Institute, NIH, 37 Convent Dr., Building 37, Room 2122C, Bethesda, MD 20892, USA, Tel: (301) 435-6315; Fax: (301) 480-2315; Email: [email protected]; or Stephen J. Savarino, U.S. Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA, Tel: (301) 319-7650; Fax: (301) 319-7679; Email: [email protected]
منابع مشابه
Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli.
In the intestine, enterotoxigenic Escherichia coli works against peristaltic forces, adhering to the epithelium via the colonization factor antigen I (CFA/I) fimbrial adhesin CfaE. The CfaE adhesin is similar in localization and tertiary (but not primary) structure to FimH, the type 1 fimbrial adhesin of uropathogenic E. coli, which shows shear-dependent binding to epithelial receptors by an al...
متن کاملAdaptive evolution of class 5 fimbrial genes in enterotoxigenic Escherichia coli and its functional consequences.
Class 5 fimbriae of enterotoxigenic Escherichia coli (ETEC) comprise eight serologically discrete colonization factors that mediate small intestinal adhesion. Their differentiation has been attributed to the pressure imposed by host adaptive immunity. We sequenced the major pilin and minor adhesin subunit genes of a geographically diverse population of ETEC elaborating CFA/I (n = 31), CS17 (n =...
متن کاملThe major subunit, CfaB, of colonization factor antigen i from enterotoxigenic Escherichia coli is a glycosphingolipid binding protein.
Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed of a major repeating subunit, CfaB, and a single tip subunit, CfaE. The potent...
متن کاملBinding of lactoferrin and free secretory component to enterotoxigenic Escherichia coli.
The ability of two glycoproteins of human milk, lactoferrin and free secretory component, to bind to Escherichia coli colonization factors (CFAs) was investigated using immunocytochemistry assays of enriched fimbrial extracts. The results revealed that lactoferrin binds to fimbrial CFA I adhesin but not to CFA II adhesin (CS1 and CS3), while free secretory component interacts with both CFA I an...
متن کاملCrystallization and preliminary X-ray diffraction analyses of several forms of the CfaB major subunit of enterotoxigenic Escherichia coli CFA/I fimbriae
Enterotoxigenic Escherichia coli (ETEC), a major global cause of diarrhea, initiates the pathogenic process via fimbriae-mediated attachment to the small intestinal epithelium. A common prototypic ETEC fimbria, colonization factor antigen I (CFA/I), consists of a tip-localized minor adhesive subunit CfaE and the stalk-forming major subunit CfaB, both of which are necessary for fimbrial assembly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2007